旋毛虫
生物
微生物学
组织蛋白酶
寄主(生物学)
肠粘膜
组织蛋白酶L
组织蛋白酶B
免疫学
蠕虫
酶
生物化学
生态学
医学
内科学
作者
You Jiao Zao,Guangcun Cheng,Miao Miao Feng,Yi Xuan Wang,Zi Fang Zhang,Xi Zhang,Peng Jiang
标识
DOI:10.1016/j.ijbiomac.2023.128728
摘要
Trichinellosis is a zoonotic parasitic disease that poses threats to human health, the meat industry, food safety, and huge financial losses. The critical stage of Trichinella spiralis (T. spiralis) infection is the invasion of intestinal larvae into the host's intestinal epithelial cells (IECs). T. spiralis Cathepsin B (TsCB) specifically interacts with IECs to facilitate the invasion of larvae. This study aims to look at how TsCB affects mouse IECs. TsCB was successfully cloned, expressed, and characterized, demonstrating its natural cysteine protease hydrolysis activity. A total of 140 proteins that interact with rTsCB were identified by GST pull-down combined with LC-MS/MS, including type I collagen, an essential component of the host's intestinal epithelial barrier system and intimately related to intestinal epithelial damage. TsCB transcription and expression levels rise, whereas type I collagen in the host's intestinal mucosa declines when the T. spiralis larvae invaded. Besides, it was discovered that TsCB bound to and degraded type I collagen of the host's intestine. This research can serve as a foundation for clarifying how T. spiralis invades the host's intestinal barrier and might provide information on potential targets for the creation of novel treatments to treat parasite illnesses.
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