A Highly Bright Near‐Infrared Afterglow Luminophore for Activatable Ultrasensitive In Vivo Imaging

Abstract Afterglow luminescence imaging probes, with long‐lived emission after cessation of light excitation, have drawn increasing attention in biomedical imaging field owing to their elimination of autofluorescence. However, current afterglow agents always suffer from an unsatisfactory signal intensity and complex systems consisting of multiple ingredients. To address these issues, this study reports a near‐infrared (NIR) afterglow luminophore (TPP‐DO) by chemical conjugation of an afterglow substrate and a photosensitizer acting as both an afterglow initiator and an energy relay unit into a single molecule, resulting in an intramolecular energy transfer process to improve the afterglow brightness. The constructed TPP‐DO NPs emit a strong NIR afterglow luminescence with a signal intensity of up to 108 p/s/cm2/sr at a low concentration of 10 μM and a low irradiation power density of 0.05 W/cm2, which is almost two orders of magnitude higher than most existing organic afterglow probes. The highly bright NIR afterglow luminescence with minimized background from TPP‐DO NPs allows a deep tissue penetration depth ability. Moreover, we develop a GSH‐activatable afterglow probe (Q‐TPP‐DO NPs) for ultrasensitive detection of subcutaneous tumor with the smallest tumor volume of 0.048 mm3, demonstrating the high potential for early diagnosis and imaging‐guided surgical resection of tumors.