生物
衰老
表观遗传学
DNA甲基化
体细胞
细胞生物学
电池类型
遗传学
端粒
细胞
基因
基因表达
作者
Katarzyna Malgorzata Kwiatkowska,Eleni Mavrogonatou,Adamantia Papadopoulou,Claudia Sala,Luciano Calzari,Davide Gentilini,Maria Giulia Bacalini,Daniele Dall’Olio,Gastone Castellani,Francesco Ravaioli,Claudio Franceschi,Paolo Garagnani,Chiara Pirazzini,Dimitris Kletsas
出处
期刊:Cells
[MDPI AG]
日期:2023-03-17
卷期号:12 (6): 927-927
被引量:4
标识
DOI:10.3390/cells12060927
摘要
The aim of the present study was to provide a comprehensive characterization of whole genome DNA methylation patterns in replicative and ionizing irradiation- or doxorubicin-induced premature senescence, exhaustively exploring epigenetic modifications in three different human cell types: in somatic diploid skin fibroblasts and in bone marrow- and adipose-derived mesenchymal stem cells. With CpG-wise differential analysis, three epigenetic signatures were identified: (a) cell type- and treatment-specific signature; (b) cell type-specific senescence-related signature; and (c) cell type-transversal replicative senescence-related signature. Cluster analysis revealed that only replicative senescent cells created a distinct group reflecting notable alterations in the DNA methylation patterns accompanying this cellular state. Replicative senescence-associated epigenetic changes seemed to be of such an extent that they surpassed interpersonal dissimilarities. Enrichment in pathways linked to the nervous system and involved in the neurological functions was shown after pathway analysis of genes involved in the cell type-transversal replicative senescence-related signature. Although DNA methylation clock analysis provided no statistically significant evidence on epigenetic age acceleration related to senescence, a persistent trend of increased biological age in replicative senescent cultures of all three cell types was observed. Overall, this work indicates the heterogeneity of senescent cells depending on the tissue of origin and the type of senescence inducer that could be putatively translated to a distinct impact on tissue homeostasis.
科研通智能强力驱动
Strongly Powered by AbleSci AI