化学
检出限
荧光
散射
分析化学(期刊)
碱性磷酸酶
信号(编程语言)
色谱法
光学
生物化学
物理
程序设计语言
计算机科学
酶
作者
Lide Yao,Shan He,Yiyu Chen,Huiqin Lian,Bin Liu,Lai Chen,Xiaofeng Wei
出处
期刊:Talanta
[Elsevier]
日期:2023-12-01
卷期号:265: 124863-124863
标识
DOI:10.1016/j.talanta.2023.124863
摘要
Abnormal expression of alkaline phosphatase (ALP) in serum has received considerable attention in health monitoring and disease diagnosis. However, conventional optical analysis based on a single signal must compromise background interference and limited sensitivity in trace analysis. As an alternative candidate, the ratiometric approach depends on the self-calibration of two independent signals in a single test to minimize interferences from the background for accurate identification. Here, a carbon dot/cobalt-metal organic framework nanocoral (CD/Co-MOF NC) mediated fluorescence-scattering ratiometric sensor has been developed for simple, stable, and highly sensitive detection of ALP. ALP-responsive phosphate production was used to coordinate cobalt ion and collapse the CD/Co-MOF NC, resulting in the recovery of fluorescence signal from dissociative CDs and the decrease of second-order scattering (SOS) signal from the cracked CD/Co-MOF NC. The ligand-substituted reaction and the optical ratiometric signal transduction provide a rapid and reliable chemical sensing mechanism. The ratiometric sensor effectively converted ALP into a ratio signal of fluorescence-scattering dual emission throughout a wide linear concentration range of six orders of magnitude with a detection limit of 0.6 mU/L. In addition, self-calibration of fluorescence-scattering ratiometric method can reduce background interference and improve sensitivity in serum, approaching recoveries of ALP from 98.4% to 101.8%. Due to the above advantages, the CD/Co-MOF NC mediated fluorescence-scattering ratiometric sensor readily provides rapid and stable quantitative detection of ALP as a promising in vitro analytical method for clinical diagnostics.
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