玉米秸秆
水解物
棕榈油酸
食品科学
干草
发酵
化学
木糖
脂肪酸
生物化学
酵母
生物
生物技术
农学
作物
水解
棕榈酸
作者
Shaozheng Li,Changsheng Su,Mudannan Fang,Di Cai,Li Deng,Fang Wang,Junfeng Liu
标识
DOI:10.1016/j.biortech.2023.129211
摘要
Palmitoleic acid (POA) has been widely applied to nutrition and pharmaceutical industry. However, high cost of scale-up fermentation restricts the extensive application of POA. Hence, we investigated the availability of corn stover hydrolysate (CSH) as carbon source in POA production by engineered S. cerevisiae. Although the yeast growth was inhibited to some extent by CSH, the POA production with CSH was slightly higher than that with pure glucose. The C/N ratio of 120 and addition of 1 g/L lysine raised the POA titer up to 2.19 g/L and 2.05 g/L, respectively. Two-stage cultivation could increase the POA titer by upregulating the gene expression of key enzymes in fatty acid synthesis pathway. A high POA content of 57.5% (v/v) and a highest POA titer of 6.56 g/L were achieved under the optimized conditions. These findings provide a feasible approach for sustainable production of POA or its derivatives from CSH.
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