The application of zinc oxide nanoparticles: An effective strategy to protect rice from rice blast and abiotic stresses

The causal agent of blast disease, the filamentous fungus Magnaporthe oryzae, leads to tremendous damage on rice production worldwide. Zinc oxide nanoparticles (ZnO NPs) have multi-functions in plant growth and antimicrobial activity. However, the effects of ZnO NPs on M. oryzae and disease resistance in rice are still unclear. Here, we showed that ZnO NPs have direct antifungal activity against M. oryzae by inhibiting its conidiation and appressorium formation. In addition, ZnO NPs significantly inhibit blast development and enhance basal resistance in rice by inducing ROS accumulation and expression of defense-related genes OsNAC4, OsPR10, OsKSL4, and OsPR1b. Furthermore, we showed that ZnO NPs treatment reduces ABA level in plant, leading to increased ROS accumulation and enhanced resistance against M. oryzae. Importantly, ZnO NPs treatment improves the tolerance of rice seedlings to osmotic and heat stresses.In conclusion, not only being an effective aid in fighting against blast disease, ZnO NPs also provides a novel strategy to enhance the tolerance of rice seedlings to abiotic stress.