Single-shot phosphorescence lifetime imaging thermometry for wide-field temperature sensing

Photoluminescence lifetime imaging of upconverting nanoparticles (UCNPs) is useful for optical thermometry but has not yet been implemented in wide-field temperature sensing in a single exposure. To overcome this limitation, we have developed single-shot photoluminescence lifetime imaging thermometry (SPLIT). Using the core/shell structured UCNPs as the temperature indicators, we apply SPLIT to wide-field temperature mapping of a dynamic biological sample at video rate.