化学发光
纳米团簇
检出限
鲁米诺
过氧化物酶
化学
淀粉
淀粉酶
放射性检测
色谱法
生物化学
酶
有机化学
计算机科学
人工智能
作者
Xiaoxu Zhang,Yuying Jia,Yanqun Fei,Yongzhuang Lu,Xiaoli Liu,Hongyan Shan,Yanfu Huan
出处
期刊:Analytical Methods
[The Royal Society of Chemistry]
日期:2023-01-01
卷期号:15 (12): 1553-1558
被引量:3
摘要
Herein, a novel chemiluminescence method was developed for efficient and sensitive detection of α-amylase activity. α-Amylase is closely related to our life, and α-amylase concentration is a marker for the diagnosis of acute pancreatitis. In this paper, Cu/Au nanoclusters with peroxidase-like activity were prepared using starch as a stabilizer. Cu/Au nanoclusters can catalyze H2O2 to generate reactive oxygen species and increase the CL signal. The addition of α-amylase makes the starch decompose and causes the nanoclusters to aggregate. The aggregation of the nanoclusters caused them to increase in size and decrease in the peroxidase-like activity, resulting in a decrease in the CL signal. α-Amylase was detected by the CL method of signal changes caused by dispersion-aggregation in the range of 0.05-8 U mL-1 with a low detection limit of 0.006 U mL-1. The chemiluminescence scheme based on the luminol-H2O2-Cu/Au NC system is of great significance for the sensitive and selective determination of α-amylase in real samples, and the detection time is short. This work provides new ideas for the detection of α-amylase based on the chemiluminescence method and the signal lasts for a long time, which can realize timely detection.
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