移相器
纳米孔测序
模块化设计
纳米孔
计算机科学
图形
顺序装配
计算生物学
纳米技术
工程类
基因组
生物
理论计算机科学
材料科学
遗传学
程序设计语言
基因
电气工程
基因表达
转录组
作者
Ryan Lorig-Roach,Melissa Meredith,Jean Monlong,Miten Jain,Hugh E. Olsen,Brandy M. McNulty,David Porubský,Tessa G. Montague,Julian Lucas,Chris Condon,Jordan Eizenga,Sissel Juul,Sean K. McKenzie,Sara Simmonds,Jimin Park,Mobin Asri,Sergey Koren,Evan E. Eichler,Richard Axel,Bruce Martin,P. Carnevali,Karen H. Miga,Benedict Paten
标识
DOI:10.1101/2023.02.21.529152
摘要
As a step towards simplifying and reducing the cost of haplotype resolved de novo assembly, we describe new methods for accurately phasing nanopore data with the Shasta genome assembler and a modular tool for extending phasing to the chromosome scale called GFAse. We test using new variants of Oxford Nanopore Technologies' (ONT) PromethION sequencing, including those using proximity ligation and show that newer, higher accuracy ONT reads substantially improve assembly quality.
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