阿特拉津
内分泌系统
内分泌学
细胞生物学
内科学
化学
生物
激素
医学
生态学
杀虫剂
作者
Xiaowei Li,Kai Guo,Chi Chiu Wang,Yang Yu,Wei Li,Milton Talukder,Xue-Nan Li,Jinlong Li
标识
DOI:10.1016/j.psj.2024.103730
摘要
Atrazine (ATR) is widely used worldwide as a commercial herbicide, Diaminochlorotriazine (DACT) is the main metabolite of ATR in the organism. both of them disrupt the production of steroids and induce abnormal reproductive development. The granulosa cells (GCs) are important for growth and reproduction of animals. However, the toxicity of ATR on the GCs of birds is not well clarified. To evaluate the effect of the environmental pollutant ATR on bird GCs. The quail GCs were allotted into 7 groups, C (The medium of M199), A20 (20 µM ATR), A100 (100 µM ATR), A250 (250 µM ATR), D20 (20 µM DACT), D100 (100 µM DACT), D200 (200 µM DACT). The results demonstrated that ATR reduced the viability of GCs, disrupted mitochondrial structure (including mitochondrial cristae fragmentation and the mitochondrial morphology disappearance) and decreased mitochondrial membrane potential. Meanwhile, ATR interfered with the expression of key factors in the steroid synthesis pathway, inducing the secretion of the sex hormone E2 and P in GCs. which in turn induced apoptosis. Furthermore, The Nrf2/ARE pathway as a potential target to ameliorate ATR-induced endocrine disruption in GCs for proper reproductive function. Our research provides a new perspective for understanding the effects of ATR on reproductive functions in birds.
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