子宫
发情周期
移植
缺血
医学
不育
组织病理学
男科
病理
内科学
生物
怀孕
遗传学
作者
Xian Luo,Shengnan Yu,Bing Lu,Qisheng Zheng,Xin Zhou,Ke An,Jiaying Zhong,Liang Wu,Helong Dai,Zhongquan Qi,Junjie Xia
出处
期刊:Transplantation
[Ovid Technologies (Wolters Kluwer)]
日期:2024-03-19
标识
DOI:10.1097/tp.0000000000004979
摘要
Uterus transplantation (UTx) is an emerging treatment for uterine factor infertility. Determining the maximum tolerable cold ischemia time is crucial for successful UTx. However, the limit for cold ischemia in the uterus is unclear. This study aimed to examine cold ischemia's effects on mouse uteri and identify the maximum cold ischemia duration that uteri can endure.We systematically assessed the tolerance of mouse uteri to extended cold ischemia, 24 h, 36 h, and 48 h, using the cervical heterotopic UTx model. Multiple indicators were used to evaluate ischemia-reperfusion injury, including reperfusion duration, macroscopic examination, oxidative stress, inflammation, and histopathology. The function of transplants was evaluated through estrous cycle monitoring and embryo transfer.Mouse uteri subjected to 48 h of cold ischemia exhibited significant delays and insufficiencies in reperfusion, substantial tissue necrosis, and loss of the estrous cycle. Conversely, uteri that underwent cold ischemia within 36 h showed long survival, regular estrous cycles, and fertility.Our study demonstrated that mouse uteri can endure at least 36 h of cold ischemia, extending the known limits for cold ischemia and providing a pivotal reference for research on the prevention and treatment of cold ischemic injury in UTx.
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