毛囊
细胞生物学
氧化应激
伤口愈合
干细胞
MAPK/ERK通路
活性氧
细胞凋亡
细胞生长
化学
癌症研究
生物
信号转导
生物化学
免疫学
作者
Hong Niu,Hai-chang Li,Yue Guan,Xin Zhou,Zhong-Guang Li,Serana Li Zhao,Peng Chen,Tao Tan,Hua Zhu,Valerie Bergdall,Xuehong Xu,Jianjie Ma,Jianjun Guan
标识
DOI:10.1016/j.bioactmat.2022.03.017
摘要
MG53 is an essential component of the cell membrane repair machinery, participating in the healing of dermal wounds. Here we develop a novel delivery system using recombinant human MG53 (rhMG53) protein and a reactive oxygen species (ROS)-scavenging gel to treat diabetic wounds. Mice with ablation of MG53 display defective hair follicle structure, and topical application of rhMG53 can promote hair growth in the mg53-/- mice. Cell lineage tracing studies reveal a physiological function of MG53 in modulating the proliferation of hair follicle stem cells (HFSCs). We find that rhMG53 protects HFSCs from oxidative stress-induced apoptosis and stimulates differentiation of HSFCs into keratinocytes. The cytoprotective function of MG53 is mediated by STATs and MAPK signaling in HFSCs. The thermosensitive ROS-scavenging gel encapsulated with rhMG53 allows for sustained release of rhMG53 and promotes healing of chronic cutaneous wounds and hair follicle development in the db/db mice. These findings support the potential therapeutic value of using rhMG53 in combination with ROS-scavenging gel to treat diabetic wounds.
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