基因敲除
花粉管
花粉
基因
生物
计算生物学
细胞生物学
遗传学
植物
授粉
作者
Martin Potocký,Radek Bezvoda,Přemysl Pejchar
出处
期刊:Methods in molecular biology
日期:2019-01-01
卷期号:: 359-365
被引量:1
标识
DOI:10.1007/978-1-4939-9469-4_24
摘要
Specific gene knockdown mediated by the antisense oligodeoxynucleotides (AODNs) strategy emerged as a rapid and effective tool for probing gene role in plant cells, particularly tip-growing pollen tubes. Here, we describe the protocol for the successful employment of AODN technique in growing tobacco pollen tubes, covering AODN design, application, and analysis of the results. We also discuss the advantages and drawbacks of this method.
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