次氯酸钠
二氧化氯
大肠杆菌
化学
钠
微生物学
氯
次氯酸盐
大肠杆菌
食品科学
无机化学
生物化学
生物
有机化学
基因
作者
David F. Bridges,Alison Lacombe,Vivian C.H. Wu
标识
DOI:10.3389/fmicb.2022.923964
摘要
Chlorine dioxide (ClO 2 ) and sodium hypochlorite (NaClO) are two chlorinated oxidizing agents that are implemented in water treatment and postharvest processing of fresh produce. While the antibacterial mechanisms of NaClO have been investigated, there are comparatively few studies that have looked at how ClO 2 kills bacteria. Therefore, the objective of this study was to compare the inactivation pathways of ClO 2 and NaClO against Escherichia coli O157:H7. Treatments consisted of 2.5, 5, and 10 ppm ClO 2 or 50, 100, and 200 ppm NaClO for 5, 10, and 15 min. Maximum log reductions of E. coli O157:H7 were 5.5 and 5.1 after treatment with ClO 2 or NaClO, respectively. Bacterial inactivation was measured using log reductions, intracellular reactive oxygen species (ROS) using with 2′,7′–dichlorofluorescin diacetate (DCFDA) or aminophenyl fluorescein (APF) probes, relative values of NAD + , NADH, NADP + , and NADPH cofactors. Additionally, the expression of three key genes involved in ROS stress was measured via RT-PCR. Levels of intracellular ROS measured by DCFDA after ClO 2 treatment were significantly higher than those found after treatment in NaClO. Additionally, NaClO treatment resulted in upregulation of ROS-defense genes, while expression of the same genes was typically at base levels or downregulated after ClO 2 treatment. As the concentrations of both treatments increased, the NADP + :NADPH ratio shifted to the cofactor being predominantly present as NADP + . These data indicate that ClO 2 and NaClO damage E. coli O157:H7 via measurably different mechanisms and that ClO 2 does not appear to cause substantial oxidative stress to E. coli O157:H7 directly.
科研通智能强力驱动
Strongly Powered by AbleSci AI