紧密连接
脊髓损伤
细胞生物学
封堵器
血管通透性
内皮干细胞
血管生成
化学
医学
免疫学
病理
作者
Yang Luo,Fei Yao,Xuyang Hu,Yiteng Li,Yihao Chen,Ziyu Li,Zhenyu Zhu,Shuisheng Yu,Dasheng Tian,Li Cheng,Meige Zheng,Juehua Jing
标识
DOI:10.1016/j.brainresbull.2021.12.019
摘要
After spinal cord injury (SCI), endogenous angiogenesis occurs in the injury core, unexpectedly accompanied by continuous leakage of the blood-spinal cord barrier (BSCB), which may be caused by destruction of the tight junctions (TJs) between vascular endothelial cells—an important structure of the BSCB. Blood-derived macrophages infiltrate into the spinal cord, aggregate to the injury core and then polarize toward M1/M2 phenotypes after SCI. However, the effect of macrophages with different polarizations on the TJs between vascular endothelial cells remains unclear. Here, we demonstrated that from 7 days postinjury (dpi) to 28 dpi, accompanied by the aggregation of macrophages, the expression of claudin-5 (CLN-5) and zonula occludens-1 (ZO-1) in vascular endothelial cells in the injury core was significantly decreased in comparison to that in normal spinal cord tissue and in the penumbra. Moreover, the leakage of the BSCB was severe in the injury core, as demonstrated by FITC-dextran perfusion. Notably, our study demonstrated that depletion of macrophages facilitated the restoration of TJs between vascular endothelial cells and decreased the leakage of BSCB in the injury core after SCI. Furthermore, we confirmed that the endothelial TJs could be impaired by M1 macrophages through secreting IL-6 in vitro, leading to an increased permeability of endothelial cells, but it was not significantly affected by M0 and M2 macrophages. These results indicated that the TJs between vascular endothelial cells were impaired by M1 macrophages in the injury core, potentially resulting in continuous leakage of the BSCB after SCI. Preventing M1 polarization of macrophages or blocking IL-6 in the injury core may promote restoration of the BSCB, thus accelerating functional recovery after SCI. ● The endothelial TJs are impaired in the injury core at the chronic phase of SCI. ● M1 macrophages impair the endothelial TJs by secreting IL-6 after SCI. ● Myelin-laden macrophages impair the TJs while M2 macrophages protect them in vitro.
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