免疫沉淀
谷胱甘肽S-转移酶
融合蛋白
DNA损伤
转移酶
化学
细胞生物学
蛋白质-蛋白质相互作用
调节器
DNA
生物
谷胱甘肽
分子生物学
生物化学
基因
酶
重组DNA
作者
Amanda L. Bain,Janelle L. Harris,Kum Kum Khanna
出处
期刊:Methods in molecular biology
日期:2017-01-01
卷期号:: 163-181
被引量:5
标识
DOI:10.1007/978-1-4939-6955-5_13
摘要
The ATM kinase is a master regulator of the DNA damage response, and can interact with more than 700 proteins in response to DNA damage. These interactions play a critical role in fine-tuning the response of ATM to multiple cellular stressors, and can play both a positive or negative role in regulating its activity. Here, we detail using protein-protein interaction methods, including co-immunoprecipitation and Glutathione-S-transferase (GST) fusion protein pull-down assays to understand the molecular interactions of ATM. These assays give valuable functional insights into the role of ATM, as they are easy to establish within the laboratory, are not overly laborious, and are easily reproducible.
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