慢病毒
遗传增强
病毒载体
计算机科学
转染
生物
载体(分子生物学)
生物技术
细胞培养
计算生物学
病毒学
基因
人类免疫缺陷病毒(HIV)
遗传学
重组DNA
病毒性疾病
作者
Alexandra McCarron,Martin Donnelley,Chantelle McIntyre,David Parsons
标识
DOI:10.1016/j.jbiotec.2016.10.016
摘要
Lentiviruses are becoming an increasingly popular choice of gene transfer vehicle for use in the treatment of a variety of genetic and acquired human diseases. As research progresses from basic studies into pre-clinical and clinical phases, there is a growing demand for large volumes of high purity, concentrated vector, and accordingly, the means to produce such quantities. Unlike other viral vectors, lentiviruses are difficult to produce using stable cell lines, therefore transient transfection of adherent cell lines is conventionally used, and this method has proven challenging to up-scale. Furthermore, with the required increases in the volume of vector needed for larger animal and human use, comes the need for more efficient and sophisticated supernatant purification and concentration techniques. This review presents the challenges of up-scaling lentivirus production and processing approaches, novel systems for overcoming these issues, and the quality assessments recommended for producing a clinical grade lentiviral gene therapy product.
科研通智能强力驱动
Strongly Powered by AbleSci AI