胞外聚合物
化学
荧光光谱法
基质(化学分析)
基质(水族馆)
色谱法
活性污泥
多糖
荧光
相(物质)
化学工程
生物化学
有机化学
生物膜
废水
细菌
物理
海洋学
量子力学
生物
工程类
遗传学
地质学
废物管理
作者
Bing‐Jie Ni,Fang Fang,Wei Xie,Min Sun,Guo‐Ping Sheng,Weihua Li,Han‐Qing Yu
出处
期刊:Water Research
[Elsevier]
日期:2009-03-01
卷期号:43 (5): 1350-1358
被引量:163
标识
DOI:10.1016/j.watres.2008.12.004
摘要
In this work the extracellular polymeric substances (EPS) produced by mixed microbial community in activated sludge are characterized using gel-permeating chromatography (GPC), 3-dimensional excitation-emission matrix (EEM) fluorescence spectroscopy measurement and mathematical modeling. Chromatograms of extracted EPS exhibit seven peaks, among which proteins have four peaks and polysaccharides have three peaks. Evolution of the chromatogram area indicates that the quantity of produced EPS increases significantly in the substrate utilization process. With the parallel factor analysis (PARAFAC) approach, two components of the polymer matrix are identified by the EEM analysis, one as EPS proteins at Ex/Em 280/340 nm and one matrix associated as fulvic-acid-like substances at 320/400 nm. The proteins and fulvic-acid-like substances in the EPS increase in the substrate utilization phase, but decrease in the endogenous phase. To have a better insight into EPS production, the kinetic modeling of EPS is performed with regard to their molecular weight distribution and chemical natures identified by GPC and EEM. In this way, the dynamics of these important microbial products are better understood.
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