坐骨神经
感觉系统
运动神经元
荧光
化学
感觉神经元
轴浆运输
解剖
神经元
神经科学
脊髓
生物
物理
量子力学
作者
C.T. Byers,Ruicheng Fan,Aurora Messina,Wayne A. Morrison,Mary P. Galea
标识
DOI:10.1016/s0165-0270(01)00520-9
摘要
We compared the efficacy with which the fluorescent tracers Fast Blue (FB) and Diamidino Yellow (DY) retrogradely label neutrons. Trace crystals were applied to the sciatic nerve exclusively (single label) or serially (double label). Unbiased cell counts showed that FB and DY label similar numbers of motoneurons (P=1.00, df 5) or DRG neurons (P=0.95, df 5) when applied exclusively. Plotting of motoneurons revealed a similar pattern of distribution of FB and DY labeled neurons. When the tracers were applied serially, 79% of labeled motoneurons and 77% of labeled DRG neurons were double-labeled irrespective of which tracer was applied first. Equal proportions of the remaining labeled neurons were single-labeled with FB or DY. These data show that FB and DY label equal numbers of motor and sensory neurons of the sciatic nerve following exclusive or serial application of tracers. These findings support the use of FB and DY together in serial fluorescent labeling experiments.
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