生物
核运输
病毒学
衣壳
单纯疱疹病毒
刺
病毒包膜
DNA病毒
病毒进入
基因组
基因
病毒复制
病毒
遗传学
细胞生物学
细胞核
航空航天工程
工程类
作者
Yu-Jin Hong,Heena Jeong,Kiwon Park,Sungwon Lee,Jae Jeong Shim,Hye Won Kim,Yang Song,Seo-Woo Park,Hyun Lee,Victor Kim,Kwangseog Ahn
出处
期刊:Proceedings of the National Academy of Sciences
日期:2021-08-17
卷期号:118 (33)
被引量:10
标识
DOI:10.1073/pnas.2108631118
摘要
Once inside the host cell, DNA viruses must overcome the physical barrier posed by the nuclear envelope to establish a successful infection. The mechanism underlying this process remains unclear. Here, we show that the herpesvirus exploits the immune adaptor stimulator of interferon genes (STING) to facilitate nuclear import of the viral genome. Following the entry of the viral capsid into the cell, STING binds the viral capsid, mediates capsid docking to the nuclear pore complex via physical interaction, and subsequently enables accumulation of the viral genome in the nucleus. Silencing STING in human cytomegalovirus (HCMV)-susceptible cells inhibited nuclear import of the viral genome and reduced the ensuing viral gene expression. Overexpressing STING increased the host cell's susceptibility to HCMV and herpes simplex virus 1 by improving the nuclear delivery of viral DNA at the early stage of infection. These observations suggest that the proviral activity of STING is conserved and exploited by the herpesvirus family. Intriguingly, in monocytes, which act as latent reservoirs of HCMV, STING deficiency negatively regulated the establishment of HCMV latency and reactivation. Our findings identify STING as a proviral host factor regulating latency and reactivation of herpesviruses.
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