Abstract Autographa californica multiple Nucleopolyhedrovirus (AcMNPV) is the archetypal species of the alphabaculovirus . Sf9 are the ovarian cells of their host —Spodoptera frugiperda . In this study, a total of 3,463 pieces of time-series differentially expressed RNA are identified, including 1,200 mRNA and 2,263 lncRNA, with high-throughput sequencing technology using samples collected from Sf9 cells at different time points after AcMNPV infection. The result could provide a reference for the further study of the interaction and regulatory mechanism between the virus and the host.