外渗
急性胰腺炎
医学
胰腺炎
病理
白细胞外渗
炎症
胰腺
渗透(HVAC)
病理生理学
微循环
胰腺疾病
免疫学
内科学
热力学
物理
作者
Eduard Ryschich,Vachtang Kerkadze,Olegas Deduchovas,Olga Salnikova,Audris Parseliunas,Angela Märten,Werner Hartwig,Markus Sperandio,Jan Schmidt
出处
期刊:Gut
[BMJ]
日期:2009-05-20
卷期号:58 (11): 1508-1516
被引量:18
标识
DOI:10.1136/gut.2008.170001
摘要
Background: Pancreatic infiltration by leucocytes represents a hallmark in acute pancreatitis. Although leucocytes play an active role in the pathophysiology of this disease, the relation between leucocyte activation, microvascular injury and haemorrhage has not been adequately addressed. Methods: We investigated intrapancreatic leucocyte migration, leucocyte extravasation and pancreatic microperfusion in different models of oedematous and necrotising acute pancreatitis in lys-EGFP-ki mice using fluorescent imaging and time-lapse intravital microscopy. Results: In contrast to the current paradigm of leucocyte recruitment, the initial event of leucocyte activation in acute pancreatitis was represented through a dose- and time-dependent occlusion of pancreatic capillaries by intraluminally migrating leucocytes. Intracapillary leucocyte accumulation (ILA) resulted in dense filling of almost all capillaries close to the area of inflammation and preceded transvenular leucocyte extravasation. ILA was also initiated by isolated exposure of the pancreas to interleukin 8 or fMLP, demonstrating the causal role of chemotactic stimuli in the induction of ILA. The onset of intracapillary leucocyte accumulation was strongly inhibited in LFA-1−/− and ICAM-1−/− mice, but not in Mac-1−/− mice. Moreover, prevention of intracapillary leucocyte accumulation led to the development of massive capillary haemorrhages and transformed mild pancreatitis into lethal haemorrhagic disease. Conclusions: ILA represents a novel protective and potentially lifesaving mechanism of haemostasis in acute pancreatitis. This process depends on expression of LFA-1 and ICAM-1 and precedes the classical steps of the leucocyte recruitment cascade.
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