大肠杆菌
生物
基因
细菌
核酸序列
遗传学
肠杆菌科
分子生物学
核苷酸
聚合酶链反应
革兰氏阴性菌
微生物学
作者
J. Chen,Mansel W. Griffiths
标识
DOI:10.1046/j.1472-765x.1998.00445.x
摘要
A PCR-based test was developed to differentiate Escherichia coli from other Gram-negative bacteria. The assay employed primers derived from the nucleotide sequences flanking the gene encoding the universal stress protein (uspA) and was found to be highly specific for E. coli. An 884 base pair (bp) specific product was amplified from all of the E. coli tested (n = 45), whereas no amplification was associated with non-E. coli Gram-negative bacteria (n = 11).
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