染色质
增强子
生物
转录因子
二价染色质
染色质免疫沉淀
谱系(遗传)
计算生物学
芯片排序
增强子rna
细胞分化
造血
细胞生物学
嘉雅宠物
遗传学
动力学(音乐)
染色质重塑
基因
基因表达
干细胞
发起人
物理
声学
作者
David Lara‐Astiaso,Assaf Weiner,Erika Lorenzo-Vivas,Irina Zaretsky,Diego Adhemar Jaitin,Eyal David,Hadas Keren‐Shaul,Alexander Mildner,Deborah R. Winter,Steffen Jung,Nir Friedman,Ido Amit
出处
期刊:Science
[American Association for the Advancement of Science (AAAS)]
日期:2014-08-22
卷期号:345 (6199): 943-949
被引量:678
标识
DOI:10.1126/science.1256271
摘要
Chromatin modifications are crucial for development, yet little is known about their dynamics during differentiation. Hematopoiesis provides a well-defined model to study chromatin state dynamics; however, technical limitations impede profiling of homogeneous differentiation intermediates. We developed a high-sensitivity indexing-first chromatin immunoprecipitation approach to profile the dynamics of four chromatin modifications across 16 stages of hematopoietic differentiation. We identify 48,415 enhancer regions and characterize their dynamics. We find that lineage commitment involves de novo establishment of 17,035 lineage-specific enhancers. These enhancer repertoire expansions foreshadow transcriptional programs in differentiated cells. Combining our enhancer catalog with gene expression profiles, we elucidate the transcription factor network controlling chromatin dynamics and lineage specification in hematopoiesis. Together, our results provide a comprehensive model of chromatin dynamics during development.
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