Tunable Thiol–Ene Photo-Cross-Linked Chitosan-Based Hydrogels for Biomedical Applications

Access to biocompatible hydrogels with tunable properties is of great interest in biomedical applications. Here we report the synthesis and characterization of a series of photo-cross-linked chitosan hydrogels from norbornene-functionalized chitosan (CS-nb) and various thiolated cross-linkers. The resulting materials were characterized by NMR, swelling ratio, rheology, SEM, and small angle neutron scattering (SANS) measurements. The hydrogels exhibited pH- and salt-dependent swelling, while the macro- and microscale properties could be modulated by the choice and degree of cross-linker or the polymer concentration. The materials could be molded in situ and loaded with small molecules that can be released overtime. Moreover, the incorporation of collagen in the hydrogels drastically improved cell adhesion, with excellent viabilities of human dermofibroblast cells on the hydrogels observed for up to 6 days, highlighting the potential use of these materials in the biomedical area.