光学
显微镜
时间分辨率
图像分辨率
斑点图案
相(物质)
激光线宽
材料科学
分辨率(逻辑)
相位恢复
激光器
物理
计算机科学
量子力学
傅里叶变换
人工智能
作者
Chenghang Zheng,Di Jin,Yanping He,Hongtao Lin,Juejun Hu,Zahid Yaqoob,Peter T. C. So,Renjie Zhou
出处
期刊:Advanced photonics
[SPIE - International Society for Optical Engineering]
日期:2020-11-26
卷期号:2 (06)
被引量:33
标识
DOI:10.1117/1.ap.2.6.065002
摘要
A new optical microscopy technique, termed high spatial and temporal resolution synthetic aperture phase microscopy (HISTR-SAPM), is proposed to improve the lateral resolution of wide-field coherent imaging. Under plane wave illumination, the resolution is increased by twofold to around 260 nm, while achieving millisecond-level temporal resolution. In HISTR-SAPM, digital micromirror devices are used to actively change the sample illumination beam angle at high speed with high stability. An off-axis interferometer is used to measure the sample scattered complex fields, which are then processed to reconstruct high-resolution phase images. Using HISTR-SAPM, we are able to map the height profiles of subwavelength photonic structures and resolve the period structures that have 198 nm linewidth and 132 nm gap (i.e., a full pitch of 330 nm). As the reconstruction averages out laser speckle noise while maintaining high temporal resolution, HISTR-SAPM further enables imaging and quantification of nanoscale dynamics of live cells, such as red blood cell membrane fluctuations and subcellular structure dynamics within nucleated cells. We envision that HISTR-SAPM will broadly benefit research in material science and biology.
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