Tunable NIR AIE-active optical materials for lipid droplet imaging in typical model organisms and photodynamic therapy

Near infrared (NIR) luminescent materials with aggregation-induced emission (AIE) features have attracted enormous attention in the areas of medical imaging and diagnostic therapeutics because of their low background fluorescence and strong tissue penetration. This study reports a series of easily synthesized AIEgen molecules that feature NIR emission. These molecules have a donor-donor-π-acceptor (D1-D2-π-A) structure with intramolecular charge transfer (ICT) character. The nature of charge transfer transition can be modified by different structures of D2, i.e. phenyl, thiophene, and furan ring. These AIEgens have high selectivity towards lipid droplets (LDs) in vitro and in vivo, such as zebrafish, Caenorhabditis elegans, and oil crop tissue. In addition, the effect of photodynamic therapy (PDT) on SMMC-7721 cells was investigated, and the results indicate that these AIEgens have potential application for PDT on cancer cells with white light illumination. This study reveals that these triphenylamine (TPA)-based AIEgens have great potential for biological imaging and preclinical applications of PDT.