A Brief Review of How to Construct an Enzyme-Based H<sub>2</sub>O<sub>2</sub> Sensor Involved in Nanomaterials

This article briefly reviews how to construct an enzyme based hydrogen peroxide sensor involving nanomaterials, which has the advantages of high efficiency, good sensitivity and selectivity, fast response time and an extended range of linearity with lower detection limit. Glucose biosensor is constructed by immobilizing glucose oxidase enzyme on the polycarbonate membrane and the protective cover is then filled with a physiological phosphate buffer, pH 7.4. The novel blocking hydrophobic membrane which is only permeable to hydrogen peroxide is used to eliminate electrochemical interferences. This constructed enzyme based H2O2 biosensor is miniaturized by the involvement of nanomaterials like carbon nanotubes, platinum nanoparticles and silver nanoparticles and it can achieve the effective microscopic detection of glucose. The introduction of nanomaterials including some pure metals (Ag, Au, Pd, Ni, Pt, and Cu), metal oxide (ZnO and TiO2), bimetallic (Au/Ag and Au/Pt) and carbon (nanotubes and graphene) nanomaterials in the construction of the enzyme based H2O2 biosensor improves its sensitivity and performance by enhancing the enzymatic activity, and allows the introduction of many new signal transduction technologies in biosensors. This review article summarizes the working principles of glucose oxidase based hydrogen peroxide sensor, importance of involving nanomaterials in biosensor manufacturing, basic characteristics and components of a biosensor, generations glucose biosensors, procedure of making hydrogen peroxide based biosensor, synthesis of nanomaterials involved in hydrogen peroxide biosensor, and finally some examples of nanomaterials which intervene in hydrogen peroxide biosensor.