大豆苷
大豆黄酮
染料木素
异黄酮素
染料木素
化学
色谱法
萃取(化学)
吸附
有机化学
生物化学
医学
内科学
作者
Chen Hsu,Shang-Ta Wang,Bo-Yuan Wu,Yi-Wen Hung,Nan‐Wei Su
出处
期刊:Food Chemistry
[Elsevier]
日期:2020-11-01
卷期号:331: 127312-127312
被引量:6
标识
DOI:10.1016/j.foodchem.2020.127312
摘要
Growing interest in the health benefits of soy isoflavones has led to research in the isolation of individual isoflavone species for further application. Herein, we develop a new strategy to isolate daidzein, genistein, daidzin and genistin in soybean. We investigated the impact of solvents used and the extraction time on the extracted isoflavone contents from soybean. A 30-min extraction with 65% aqueous methanol gave a total isoflavone yield of 345 mg/100 g soybean, the highest value among tested conditions. Further, we proposed a two-stage adsorption/desorption chromatography comprising macroporous resin and aluminium oxide to isolate isoflavone. First, HP-20 resin was used to separate the glucosidic and aglyconic forms of isoflavone, then individual species of isoflavone could be isolated using aluminium oxide by specific retention of 5-hydroxy isoflavone. This process achieved overall high recovery (82–97%) and purity (92–95%) of the four isoflavones, which confirms a high separating efficiency for isoflavones from soybean.
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