Clinical pathological analysis and expression of Trop-2 in human pulmonary adenocarcinoma and cell lines

Objective To study the expression and clinicopathological significance of human trophoblast cell-surface antigen 2 （Trop-2） in human lung adenocarcinoma tissue and cell line. Methods The expression of Trop-2 mRNA in nine cases of lung adenocarcinoma tissues and tissues adjacent to carcinoma was detected by RT：PCR. The protein expression of Trop-2 in human lung adenocarcinoma ceils was detected through immunofluorescence. The correlation between the expression of Trop-2 and the clinical pathological features was analyzed. Results The positive rate of Trop-2 expression was different in different human lung adenocarcinoma ceils, there was no expression in normal human bronchial epithelial cell line. Trop-2 mRNA was expressed in lung adenocarcinoma ceils and the protein expression of Trop-2 was mainly located in the membrane of lung adenocarcinoma ceils. The positive rate of Trop-2 in the tissue of human lung adenocarcinoma was significantly higher than that in tissue adjacent to carcinoma. The positive expression level increased along with the reducing of carcinoma tissue differentiation degree （ P 〈 0.05）. The expression of Trop-2 was not associated with age and sex of patients （ P 〉 0.05）. Conclusions Trop-2 is highly expressed in lung adenocarcinoma tissue and cell lines,which can be used as a marker for detection of malignant degree of human lung adenocarcinoma. Key words: Lung adenocarcinoma; Trop-2; Cell strain ;  Cell lines ;  Malignant tumor