Application of immunohistochemistry navigated laser capture microdissection to capture pure prolactin cells for proteomics

Objective To investigate the effect of immunohistochemistry navigated laser capture microdissection (LCM) to prepare pure targeted cells from prolactin pituitary adenoma and normal pituita-ry for proteomic research. Methods Immunohistochemistry navigated-LCM technique was employed to successfully obtain the pure cluster or even single cell from prolactnoma and normal pituitary frozen sec-tions. Results Immunohistochemistry could be achieved within 60 min, and 4 μm ultraviolet laser was ap-plied to quickly and precisely obtain pure targeted cells with optimal morphology but without interstitium pollution. Conclusion Immunohistochemistry navigated LCM was a valuable technique which can tackle the problem of tissue heterogeneity in proteomic research with high homogeneity and stable operative proce-dure. Key words: Pituitary adenoma;  Immunohistochemistry ;  Proteomics