Cas9
引导RNA
遗传学
基因组
基因沉默
核糖核酸
RNA干扰
基因表达
CRISPR干扰
作者
Jianping Zhou,Zhaohui Zhong,Hongqiao Chen,Qian Li,Xuelian Zheng,Yiping Qi,Yong Zhang
出处
期刊:Methods of Molecular Biology
日期:2019-01-01
卷期号:1917: 109-119
被引量:3
标识
DOI:10.1007/978-1-4939-8991-1_9
摘要
BACKGROUND MicroRNAs (miRNAs) are small noncoding RNAs that play important roles in plant development and stress responses. Loss-of-function analysis of miRNA genes has been traditionally challenging due to lack of appropriate knockout tools. In this chapter, we describe a method of using CRISPR-Cas9 for knocking out microRNA genes in rice by Agrobacterium-mediated transformation. We also demonstrate single-strand conformation polymorphism (SSCP) as an effective genotyping method for screening CRISPR-Cas9-induced mutations.
科研通智能强力驱动
Strongly Powered by AbleSci AI