细胞生长
癌症研究
细胞周期
体内
生物
细胞
体外
小RNA
增殖细胞核抗原
肝内胆管癌
G1期
细胞周期检查点
细胞生物学
内科学
基因
医学
遗传学
作者
Min Zhu,Chuan‐Yuan Wei,Jiajia Lin,Shuangshuang Dong,Dongmei Gao,Jie Chen,Yan Zhao,Binbin Liu
摘要
Abstract Ubiquitin‐like with PHD and ring finger domains 1 (UHRF1) is abnormally overexpressed in multiple cancers and closely correlated with tumor‐promoting effects, such as high proliferation. However, how UHRF1 functions in intrahepatic cholangiocarcinoma (ICC) has not yet been determined. Herein, we found that UHRF1 is overexpressed in ICC tissues. Downregulated UHRF1 attenuated the transition of the G1/S cell cycle and then suppressed cell proliferation in vitro and tumor growth in vivo. Moreover, upstream regulators of the UHRF1 expression were predicted, and we found that direct binding of miR‐124‐3p inhibited the UHRF1 expression. Elevated miR‐124‐3p suppressed proliferation and led to the arrest of the cell cycle. Furthermore, the expression of UHRF1 was positively correlated with PCNA. Clinically, we showed that elevated UHRF1 was associated with poor prognosis, and served as an independent prognostic factor in ICC patients. Together, these findings demonstrate that UHRF1, regulated by miR‐124‐3p, acts as a tumor promoter by promoting cell proliferation in ICC.
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