NAD+激酶
代谢组
烟酰胺腺嘌呤二核苷酸
烟酰胺单核苷酸
烟酰胺
化学
烟酰胺磷酸核糖转移酶
生物化学
烟酰胺
代谢组学
新陈代谢
液相色谱-质谱法
代谢物
串联质谱法
辅因子
酶
色谱法
质谱法
作者
Keisuke Yaku,Keisuke Okabe,Takashi Nakagawa
摘要
Nicotinamide adenine dinucleotide (NAD) is a major co-factor that mediates multiple biological processes including redox reaction and gene expression. Recently, NAD metabolism has received considerable attention because administration of NAD precursors exhibited beneficial effects against aging-related metabolic disorders in animals. Although numerous studies have reported that NAD levels decline with aging in multiple animal tissues, the pathway and kinetics of NAD metabolism in aged organs are not completely understood. To determine the NAD metabolism upon aging, we developed targeted metabolomics based on an LC/MS/MS system. Our method is simple and applicable to crude biological samples, including culture cells and animal tissues. Unlike a conventional enzymatic cycling assay, our approach can determine NAD and NADH (reduced form of NAD) by performing a single sample preparation. Further, we validated our method using biological samples and investigated the alteration of the NAD metabolome during aging. Consistent with previous reports, the NAD levels in the liver and skeletal muscle decreased with aging. Further, we detected a significant increase in nicotinamide mononucleotide and nicotinamide riboside in the kidney upon aging. The LC/MS/MS-based NAD metabolomics that we have developed is extensively applicable to biomedical studies, and the results will present innovative ideas for the aging studies, especially for that of NAD metabolism.
科研通智能强力驱动
Strongly Powered by AbleSci AI