Comparison of HPLC and UPLC methods for determination of evodiamin and rutacarpine in compound Huangliansu tablets

Objective: To compare HPLC and UPLC methods in the determination of evodiamin and rutacarpine in compound Huangliansu tablets.Methods: HPLC condition:Atlantis C18 column(4.6 mm×250 mm,5 μm),10 mmol·L-1 ammonium acetate(pH 3.5) as mobile phase A,acetonitrile as mobile phase B,gradient elution,velocity at 1 mL·min-1,detected wavelength of 240 nm,column temperature at 35 ℃;UPLC condition:Waters Acquity HSS T3 column(2.1 mm×100 mm,1.7 μm),10 mmol·L-1 ammonium acetate(pH 3.5) as mobile phase A,acetonitrile as mobile phase B,gradient elution,velocity at 0.5 mL·min-1,detected wavelength of 240 nm,column temperature at 35 ℃.Results: HPLC determination:the linear ranges of evodiamin and rutacarpine were 0.425-5.30 μg and 0.409-5.110 μg(r0.999),respectively;the average recovery(n=9) were 98.6% and 99.0%,respectively.UPLC determination:the linear ranges of evodiamin and rutacarpine were 0.106-0.743 μg and 0.102-0.715 μg(r0.999),respectively;the average recovery(n=9) were 99.1% and 99.2%,respectively.Conclusion: Comparing with HPLC method,UPLC method is relatively quicker,with higher throughput,higher sensitivity and less organic solvent.UPLC method can succeed alternative HPLC for Fructus Evodiae quality control in compound Huangliansu tablets.