Expression of RhoA, RhoB and RhoC in Lung Cancer Tissues and Their Clinical Significance

Objective To investigate changes of expressions of RhoA and RhoB and RhoC in lung cancertissues to explore the relationship of Rho subfamily with development,migration,and metastasis of lung carcinoma.MethodsThe expressions of RhoA,RhoB and RhoC mRNA and protein in four different lung cancer cell lines(LTEP-a-2,SK-MES-1,NCI-H446,and NCI-H292)were detected by real-time quantitative reverse transcriptionpolymerase chain reaction(QRT-PCR) and Western blotting, and were compared with MRC-5(control group). Theexpressions of RhoA,RhoB and RhoC protein in 45 cases of lung cancer tissue by immunocytochemistry.Results The expressions of RhoA and RhoB mRNA and protein in lung cancer cell line LTEP-a-2,SK-MES-1,NCI-H446,and NCI-H292 were significantly higher than those in control group(allP0.05),while no statisticaldifference was found among four lung cancer cell lines(allP0.05). The expressions of RhoA and RhoB protein inhuman lung cancer tissue were positive,but the levels of them were not statistically different between moderatelydifferentiated squamous carcinoma group and non-moderately differentiated squamous carcinoma group(allP0.05).The expression of RhoC protein was negative.ConclusionThe expressions of RhoA and RhoB were elevated inlung cancer,which indicates that Rho subfamily involve in the development,migration and metastasis of lungcancer.