Antitumor Metabolites from Marine-Derived Fungus Gliocladium catenulatum T31

OBJECTIVE To investigate the antitumor metabolites from marine-derived fungus Gliocladium catenulatum T31.METHODS The producing strain T31 was fermented in a rotary shaker and the bioactive metabolites in the fermentation broth were isolated through a bioassay-guided separation procedure.The structures of the bioactive compounds were determined with physicochemical and spectroscopic data.The antitumor activity against K562 cell line in vitro was assayed by MTT method,accompanied with the cell morphological observation under the light microscope.RESULTS Seven compounds were isolated from the fermentation broth of Gliocladium catenulatum T31 and identified as three sterol derivatives including ergosta-5,7,22-triene-3β-ol(1),ergosta-6,22-diene-3β-ol(2),ergosterol peroxide(3)and four anthraquinones including emodin(4),citreorosein(5),isorhodoptilometrin(6)and lunatin(7).Compound 1-7 inhibited the proliferation of K562 cells in various degrees,and compound 4-7 inhibited the growth of K562 cells with the IC50 values of 1.09,1.24,13.60 and 8.92 μmol·L-1,respectively.CONCLUSION The predominant antitumor metabolites from Gliocladium catenulatum T31 were sterol and anthraquinone derivatives.