Objective To study the chemical constituents of the total flavonoids from the ethanol(ω=0.7)extract of the whole herb of Comptosorus sibiricus Rupr..Methods The compounds were isolated by chromatography on the silica gel column and identified on the basis of physico-chemical constants and spectral analysis.Result Six compounds were isolated as kaempferol(Ⅰ), kaempferol-7-O-α-L-rhamnoside(Ⅱ), kaempferol 3-O-β-D- glucopyranoside(Ⅲ),kaempferol 7-O-β-D-glucopyranoside(Ⅳ),kaempferol 3,7-di-O-β-D-glucopyranoside(Ⅴ),kaempferol-3-O-β-D- glucopyranoside -7-O-α-L-rhamnoside(Ⅵ)respectively.Conclusion Compound Ⅱ is obtained from this plant for the first time.