Determination of horseradish peroxidase by spectrophotometry based on 4-aminobiphenyl-H_2O_2-HRP system

A new method for the determination of horseradish per ox idase (HRP) was investigated by spectrophotometry based on 4-aminobiphenyl-H 2O 2-HRP system.It was based on H 2O 2 oxidation of 4-aminobiphenyl catalyz ed by HRP.When it was used in the determination of free HRP,the linear range was 3.0× 10 -10 ～8.0×10 -8g/mL,and the detection limit was 3.0× 10 -10g/mL.