Application of HBsAg and HBcAg Immune Fluorescence Detection in Diagnosis of Hepatitis B Virus Associated Glomerulonephritis

Objective To observe the HBsAg and HBcAg expression in the renal tissue in hepatitis B virus associated glomerulonephritis(HBV-GN) . Methods We selected 50 cases of HBV-GN as research group and the other randomly selected 20 cases of non-HBV-GN(NHBV-GN) as control group and used indirect immunofluorescence assay to detect the HBsAg and HBcAg expression in the renal puncture biopsies. Results The HBsAg and HBcAg-positive particles in the HBV-GN renal tissue could be detected,with the positive rates of 70%(35/50) and 24%(12/50) ,while HBsAg and HBcAg failed to be detected in the kidney tissue of 20 cases of NHBV-GN,with a significant difference between the two groups(P0.05) . Conclusion The immune fluorescence detection of HBsAg and HBcAg is an important indicator in the diagnosis of HBV-GN. HBV plays an important role in the kidney tissue infection and replication and the pathogenesis of HBV-GN.