Through the TLC,silica gel column chromatography and HPLC purity tests,chloroform and methanol in the ratio of 9∶1 as the eluting solvent,and the silica gel adsorbents and sample ratio of 50∶1 were optimum conditions for the silica gel column chromatography. The purity of purified resveratrol powder by the silica gel column chromatography can be advanced from 39% to 99%.