Determination of Pyrogallol Using Reversed-phase HPLC and Internal Standard Method

A rapid,specific,and accurate HPLC method was developed and validated for the assay of pyrogallol. The assay involved a gradient elution of pyrogallol with a Thermo Hypersil C18column( 250 mm × 4. 6 mm,5 μm) using mobile phase composition of methanol and water( 0. 1% TFA) at a flow rate of 1. 0 mL / min. UV detection was performed at 266 nm. Ellagic acid was used as an internal standard. The retention time of pyrogallol and ellagic acid was 4. 33 and 19. 25 min,respectively. The linearity range of pyrogallol concentration was from 62. 5 to 2 000 mg / L. The fitting equation of calibration curve was y =0. 000 138 66 x + 0. 001 92( R = 0. 999 7) and a mean recovery of 103. 6% was found. The proposed method was applied for the quantitative determination of pyrogallol in pyrogallol products.