The Silico Cloning of CDC16 gene in pigs

By constructing the contigs for the express sequence tagged(EST)which were highly homologous to the human CDC16 gene(GenBank accession number:NM_-004661),the entire coding region of CDC16 gene was obtained through in silico cloning.This EST were BP436622,AJ656153,CJ022837 and BP161802.The length of the cloned cDNA was 2057 bp and 5′-untranslated region was 119 bp.The length of 3′-untranslated region was 75 bp.The length of the open reading frame(ORF)was 1863 bp.The deduced amino acid sequence was composed of 617 amino acid.It showed 97.6% and 95.3% similarity to corresponding human and mouse respectively.