Screening of total RNA extraction methods for RNA-sequences from different organs in Ziziphus jujuba

In order to obtain the methods for high-quality total RNA extraction from flower, fruit bearing branch and fruit in Ziziphus jujuba, taking Z. jujuba cv. ‘Zhongqiusucui' as materials, and the RNA extraction effects were compared using two kinds of total RNA extraction kits(Ambiogen and Autolab) and modified CTAB method based on the Autolab RNA extraction kit. The results showed that OD260/OD280 values of the RNA extracted by using the three RNA extraction methods had no significant differences, but a part of RNAs obtained by the two RNA extraction kits were degraded. The RNA mass concentrations from flower, fruit bearing branch and fruit were 126, 284 and 222 ng/μL by using the RNA extraction kit of Ambiogen; the RNA mass concentrations were 307, 402 and 266 ng/μL by using the RNA extraction kit of Autolab; and the RNA mass concentrations were 401, 417 and 296 ng/μL by using the modified CTAB method based on the Autolab RNA extraction kit, respectively. The modified CTAB method could generate high-integrity, high-concentration and high-purity RNA that could meet the request of transcriptome sequencing, compared with the two extraction kits.