In this study, copper oxide nanoparticles (CuO NPs) decorated reduced graphene oxide (RGO)-chitosan (CS) nanocomposites are prepared for reliable detection of hydrogen peroxide (H 2 O 2 ). The reduced GO-CS-copper oxide nanocomposites are synthesized by the chemical reduction method. The morphological and chemical structures of the nanocomposites are systemically evaluated by Fourier transform infrared spectral analysis (FT-IR), x-ray diffraction (XRD), scanning electron microscopy (SEM), and Raman analysis. A potential application of reduced GO-CS-copper oxide nanocomposites-modified electrode as a biosensor to monitor H 2 O 2 has been investigated. The electrochemical properties of the biosensor are investigated by cyclic voltammetry (CV). After optimizing all the experimental parameters, the reduced GO-CS-copper oxide nanocomposites on modified glassy carbon electrode (GCE) showed a good performance towards the electrocatalytic reduction of H 2 O 2 . This method is simple, cost-effective, sensitive, and also can be used for the determination of H 2 O 2 in a real water sample.