环介导等温扩增
材料科学
纳米技术
线性
严重急性呼吸综合征冠状病毒2型(SARS-CoV-2)
2019年冠状病毒病(COVID-19)
计算机科学
化学
工程类
电气工程
传染病(医学专业)
生物化学
医学
病理
DNA
疾病
作者
Mengyun Zhou,Yong Luo,Lirong Wang,Chuan Fan,Tailin Xu,Xueji Zhang
标识
DOI:10.1016/j.bios.2022.114903
摘要
The outbreak of COVID-19 has created a huge challenge to global health systems. Experience in fighting the epidemic shows that the development of a rapid and sensitive POCT diagnostic platform for SARS-CoV-2 that can be deployed in situ is crucial to contain the outbreak. Here, we have developed a portable microdroplet detection platform that integrated temperature controller and micro-stirring for high-throughput and ultrafast COVID-19 diagnosis. Such a device uses a p-n junction (PN junction) as the temperature controller to adjust the temperature in a single microdroplet independently and precisely, ensuring the amplification of reverse transcription loop-mediated isothermal amplification (RT-LAMP). Meanwhile, the platform incorporates an ultrasonic micro-stirring unit, greatly increasing the interaction between RT-LAMP molecules and accelerating the amplification. The results show good linearity over a wide linear range (1 to 10 5 copies/μL) and low LOD (0.48 copy/μL). Our method reports in only 6.1 min for high-viral load samples, and combines with sample preparation, the total detection process could be done within 30 min. Such a portable and fully integrated microdroplet molecular diagnostic platform is a promising tool for point-of-care diagnosis of COVID-19 and other infectious diseases in resource-limited settings. • A portable microdroplet array integrated platform for SARS-CoV-2 detection. • The temperature of each microdroplet can be independently and precisely controlled. • Ultrasound-induced microstreaming can enhance the RT-LAMP reaction. • The method presents a low LOD with 0.48 copy/μL and detection time in only 6.1 min. • Such fully integrated device holds promise for rapid and sensitive detection in clinical diagnosis.
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