生物传感器
纳米颗粒
化学
纳米材料
氮气
氨基酸
组氨酸
氧化酶试验
活动站点
纳米技术
酶
组合化学
材料科学
生物化学
有机化学
作者
Shuaiwen Li,Zihui Chen,Feng Yang,Chunxia Qiao,Shuqi Zhang,Wanqing Yue
出处
期刊:ACS applied nano materials
[American Chemical Society]
日期:2023-08-04
卷期号:6 (16): 14777-14785
被引量:1
标识
DOI:10.1021/acsanm.3c02243
摘要
Defect-engineered nanomaterials have been found to improve catalytic activity, making them highly desirable for biosensor preparation. Nitrogen doping is an effective method for introducing defects in nanomaterials, and amino acids containing nitrogen atoms can be used as a nitrogen source for doping nanozymes. In this study, nitrogen-doped N-Mn3O4 nanoparticles (NPs) were synthesized using various amino acids as nitrogen sources. The results indicate that N-Mn3O4 NPs doped with histidine as a nitrogen source exhibit the highest oxidase-like activity. Additionally, N-Mn3O4 NPs have a Vmax that is 5.4-fold higher and a Km that is 1/2-fold lower than Mn3O4, suggesting that histidine-doped N-Mn3O4 NPs can be used to create highly active oxidase-like enzymes. A dual-modal sensor utilizing both ratiometric fluorescence and smartphone-based colorimetry method with a smartphone has been developed for the detection of acid phosphatase, based on N-Mn3O4 NPs with high enzymatic activity. This study presents an approach to constructing highly active oxidase-like and realizing a highly sensitive assay for detecting acid phosphatase with high interference resistance. The mechanisms underlying the improved performance of nitrogen-doped nanomaterials and their potential applications in biosensor fields were also discussed.
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