糖萼
聚糖
硫酸乙酰肝素
酶
化学
生物化学
细胞生物学
糖胺聚糖
体外
荧光显微镜
生物物理学
生物
荧光
糖蛋白
物理
量子力学
作者
Jinhua Wei,Dongdong Liu,Tingting Xu,Limeng Zhu,Siming Jiao,Xubing Yuan,Zhuo A. Wang,Jianjun Li,Yuguang Du
标识
DOI:10.1016/j.ijbiomac.2023.126627
摘要
Glycocalyx dysfunction is believed as the first step in diabetic vascular disease. However, few studies have systematically investigated the influence of HG on the glycocalyx as a whole and its major constituent glycans towards one type of cell. Furthermore, most studies utilized traditional two-dimensional (2D) cultures in vitro, which can't provide the necessary fluid environment for glycocalyx. Here, we utilized vascular glycocalyx on chips to evaluate the changes of glycocalyx and its constituent glycans in HG induced HUVECs. Fluorescence microscopy showed up-regulation of hyaluronan (HA) but down-regulation of heparan sulfate (HS). By analyzing the metabolic enzymes of both glycans, a decrease in the ratio of synthetic/degradative enzymes for HA and an increase in that for HS were demonstrated. Two substrates (UDP-GlcNAc, UDP-GlcA) for the synthesis of both glycans were increased according to omics analysis. Since they were firstly pumped into Golgi apparatus to synthesize HS, less substrates may be left for HA synthesis. Furthermore, the differential changes of HA and HS were confirmed in vessel slides from db/db mice. This study would deepen our understanding of impact of HG on glycocalyx formation and diabetic vascular disease.
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