生物
突变体
类胡萝卜素
花青素
转录组
植物
叶绿素
颜料
基因
兰科
叶绿素b
小桶
野生型
遗传学
基因表达
化学
有机化学
作者
Xiu-Xian Ye,Yi-Quan Chen,WU Jian-she,Huai-Qin Zhong,Bing Lin,Minling Huang,Rong-Hui Fan
标识
DOI:10.1007/s10528-023-10432-7
摘要
Anoectochilus roxburghii (Wall.) Lindl is a perennial herb of the Orchidaceae family; a yellow–green mutant and a yellow mutant were obtained from the wild type, thereby providing good material for the study of leaf color variation. Pigment content analysis revealed that chlorophyll, carotenoids, and anthocyanin were lower in the yellow–green and yellow mutants than in the wild type. Transcriptome analysis of the yellow mutant and wild type revealed that 78,712 unigenes were obtained, and 599 differentially expressed genes (120 upregulated and 479 downregulated) were identified. Using the Kyoto Encyclopedia of Genes and Genomes pathway analysis, candidate genes involved in the anthocyanin biosynthetic pathway (five unigenes) and the chlorophyll metabolic pathway (two unigenes) were identified. Meanwhile, the low expression of the chlorophyll and anthocyanin biosynthetic genes resulted in the absence of chlorophylls and anthocyanins in the yellow mutant. This study provides a basis for similar research in other closely related species.
科研通智能强力驱动
Strongly Powered by AbleSci AI