Catfish is one type of fish that is widely cultivated by fish farmers because it has the ability to adapt to any kind of water condition. In the process of catfish farming, farmers must take care of catfish seeds first. In caring for catfish seedlings, breeders can provide feed in the form of microbes for these catfish seeds and breeders must separate catfish seedlings with age differences to prevent cannibalism because catfish have cannibalistic nature. Subur Jaya Farm is one of the farms in the Tambun area, West Bekasi. At this farm, the current process of separating catfish seeds is carried out in three stages, namely by transferring all catfish seeds into a cleaner container, then farmers will see one by one the size of the catfish seeds because the age of the catfish seeds can be seen from their size. Finally, farmers can move catfish seeds of the same size to a new place. However, this process takes a lot of time and has the potential to cause errors in the separation by farmers because they are not careful when looking at the size of the catfish seeds. Therefore, to overcome these problems, this study will apply Yolo V3 method to detect the age of catfish seedlings based on size. The results of this study showed an average accuracy value of 84% in the testing process. The highest accuracy value was 99% for 6 catfish seeds and the lowest was 26% for 7 catfish seedlings.